TOR-SnRK1 are modulated by upstream signaling to regulate growth and development in vitro of ‘Myrobalan 29C’ plum rootstock

Abstract The aim of this study was: (i) understand how upstream signaling modulated TOR-SnRK1 nexus; and (ii) establish an interplay between SnRK1-TOR nexus, sugar availability, sucrolytic enzyme activities, expression level key genes related to signalling metabolism, including trehalose, in vitro-grown ‘Myrobalan 29C’plum rootstock (Prunus cerasifera). Explants were cultivated Murashigue Skoog medium (MS) with trehalose (0; 1,0 10 mM). In 3 days, the antagonistic role PcSnRK1 PcTOR was confirmed plants treated 10mM possibly indicating that 29C’ not a stress condition. Furthermore, PcTREA up-regulation observed, which can lead glucose accumulation, turn is precursor sorbitol synthesis. Regarding growth parameters evaluated after 21 days vitro culture, uppermust number shoots explant length observed at trehalose. Such positive response may be due increase Glucose UDP-Glc content, direct products sucrose synthase (SuSy) enzyme. Consistent these results, highest availability molecules signal for TOR-activation. Interestingly, same condition, accumulation also have contributed ameliorate parameters.